|Year : 2020 | Volume
| Issue : 4 | Page : 240-248
Assessment of single- and double-strand breaks in DNA induced by auger electrons of radioisotopes used in diagnostic and therapeutic applications
Mahdi Seifi Moradi, Babak Shirani Bidabadi
Department of Nuclear Engineering, Faculty of Physics, University of Isfahan, Isfahan, Iran
|Date of Submission||31-Aug-2019|
|Date of Decision||02-Dec-2020|
|Date of Acceptance||02-Dec-2020|
|Date of Web Publication||2-Feb-2021|
Dr. Babak Shirani Bidabadi
Faculty of Physics, University of Isfahan, Isfahan
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Introduction: Most of the radionuclides that are used for diagnostic purposes emit Auger electrons and can thus cause damage to the DNA molecule on a nanometer scale. Therefore, the nanodosimetric calculation of these radioisotopes is necessary to achieve better understanding on their effects. Aim: The aim of this study was to calculate the mean number of DNA strand breaks (single-strand breaks and double-strand breaks) caused by direct and indirect effects for six widely used Auger electron-emitting diagnostic radioisotopes, including 123I, 125I, 99mTc, 67Ga, 201Tl, 111In and two therapeutic radioisotopes of 131I(beta + Auger + CK emitter) and 211At(alpha + Auger + CK emitter). Materials and Methods: Geant4-DNA simulation tool was used to evaluate the effects of Auger electrons, beta and alpha particles of these radioisotopes on DNA molecules. Two different DNA molecule geometric models were simulated and the results of these two models were compared with each other as well as with the results of previous studies. Results and Conclusion: The results showed that the geometric shape of the sugar-phosphate groups may have a significant effect on the number of single-strand breaks (SSBs) and double-strand breaks (DSBs) of the DNA molecule. Among the most widely used diagnostic radioisotopes, 201Tl and 125I, had the greatest impact on the number of SSBs and DSBs, respectively, while therapeutic radioisotope of 131I almost had no effect, therapeutic radioisotope of 211At had the moderate effect on the number of breaks in the DNA chain.
Keywords: Auger electron, DNA molecule, double-strand breaks, Geant4-DNA, single-strand breaks
|How to cite this article:|
Moradi MS, Bidabadi BS. Assessment of single- and double-strand breaks in DNA induced by auger electrons of radioisotopes used in diagnostic and therapeutic applications. J Med Phys 2020;45:240-8
|How to cite this URL:|
Moradi MS, Bidabadi BS. Assessment of single- and double-strand breaks in DNA induced by auger electrons of radioisotopes used in diagnostic and therapeutic applications. J Med Phys [serial online] 2020 [cited 2021 Jun 14];45:240-8. Available from: https://www.jmp.org.in/text.asp?2020/45/4/240/308614
| Introduction|| |
A number of widely-used diagnostic radioisotopes are Auger electron-emitter, most of which, due to having short range in tissue; from a few nanometers to micrometers; low energy; and medium LET, can have significant effects on living cells on a nanoscale. Considering the above characteristics, these radioisotopes can produce high levels of toxicity in cancer cells and are therefore suitable for use in molecularly targeted radiotherapy (MTRT)., Due to the short range of these electrons and their energy transfer at the site of decay, there is minimal irradiation of healthy cells adjacent to cancerous cells and therefore absorbed dose of healthy cells decreases.
A number of radioisotopes used in nuclear medicine have been proposed for molecular radiotherapy of some small metastases in cancer cells.,, 123I Radioisotope, as an Auger electron emitter, can be used to treat certain types of cancer. For this purpose, IdUrd drug (Thymidine analogue 5-iodo-2'-deoxyuridine), which carries 123I and will be located on the thymidine base of DNA molecule was used by Stigbrand et al., and Pomplun.
Watanabe et al., and Faraggi et al., also confirmed that Auger electron emitters have the potential for targeted-radiotherapy when they are located near the target site. Internal conversion electrons (even when decay occurs in the nucleoplasm) may also reach the cell's nucleus and cause damage to the DNA molecule Faraggi et al. They calculated the dose rate of a number of Auger electron-emitting radionuclides on a micrometer scale and in spherical cells with size of large lymphocytes. They concluded that the dose rate is highly dependent on the intracellular distribution of radioisotopes. When 99mTc, 123I, 111In, 67Ga, and 201Tl radioisotopes are distributed in the cell nucleus, the rate of absorbed dose at the nucleus is 94, 21, 18, 74, and 76 times higher than the state in which these radioisotopes are distributed in the cell membrane.
In addition to having the potential to be used in cell treatment, Auger electron-emitting diagnostic radioisotopes may also damage the DNA molecule because of their short-range. Therefore, it is necessary to make a comprehensive assessment of their effects and their damage to the DNA. The DNA molecule is composed of two strands running opposite to each other and connected by hydrogen bonding and forming a binary spiral structure. Each strand is a linear chain of 4 bases of adenine, cytosine, guanine and thymine linked by sugar molecules and forming sugar-phosphate backbone.
Two major damages that can occur in the DNA molecule include single-strand break (SSB) and double-strand break (DSB)., These breaks can be due to the direct ionization of the DNA molecule by ionizing radiation (direct effects) or by the interaction of water-based active radicals with the DNA molecule (indirect effects).
Tounekti et al., studied the relationship between types of cell death and number of SSBs and DSBs of DNA molecules for Chinese hamster fibroblasts using different experimental endpoints and applying bleomycin (Bleomycin) and deglyco-bleomycin antibiotics, which induced SSBs and DSBs in DNA molecules. They considered three types of cell death, namely apoptosis, mitotic cell death as pseudoapoptosis as well as repair for cells, according to the type and number of strand breaks of the DNA molecule. For example, they concluded that cell death can occur as apoptosis in case of 150000 <SSBs and 500 <DSBs; in contrast, if the number of SSBs and DSBs is smaller than 150,000 and 500, respectively, cell death does not occur and the repair process occurs instead. These threshold values for other cells can vary, which depends on the potential for DNA repair of other cells, and these thresholds cannot be used with certainty for all cells.
In a study, Pomplun used MOCA8b code to evaluate DNA molecule chain breaks caused by direct effects of decay of 123I and 125I radioisotopes. He calculated Auger electron-induced the number of SSBs and DSBs caused by 123I as 1.1 and 0.2, and by 125I as 1.9 and 0.4, per decay respectively. So far, different geometric models and various Monte Carlo codes have been used to evaluate the DNA chain breaks caused by the Auger electrons. In previous studies, linear DNA models in the form of small cylinders (smaller than 100 nm) representing duplex, nucleosome, and chromatin fibers of the DNA molecule have been used to calculate the absorbed energy.
Nikjoo et al., traced the Auger electrons produced by the characteristic soft X-rays of C (278 eV), Al (1487 eV) and Ti (4509 eV) elements, using the Monte Carlo technique (MOCA8b code) and calculated the amount of absorbed energy in cylinders with a diameter of 1-100 nm. The results showed that for a constant diameter, by increasing in cylinder length, the amount of absorbed energy increased slowly. A comparison was also made between the absorption of the energy produced by the above-mentioned characteristic X-rays with the energies of 1.2 MeV and 300 kV particle beams (particles with high LET) and 100 kV electron beams (particles with low LET) in small target volumes. The results showed that characteristic soft X-rays are more effective in comparison with other high-and low-LET radiations in transmitting and absorbing the energy in nano-sized volumes.
The volumetric DNA model was developed by Charlton and Humm. This model was simulated in a cylindrical shape with a diameter of 2.3 nm, divided into three smaller cylindrical volumes. One of these volumes was a central cylinder of diameter of 1 nm, indicating the volume occupied by base pairs. Two other volumes included semi-cylindrical shells of height of 0.34 nm, internal radius of 0.5 nm, and external radius of 1.5 nm, which surrounds the central cylinder and represents the volume occupied by sugar-phosphate backbone. Ftacnikova and Bohm and Humm and Charlton used this model to count the DNA molecule breaks. In their study, Ftacnikova and Bohm calculated the number of SSBs caused by direct effects of the Auger electrons per decay using the ETRACK code for the 123I, 125I, 67Ga, 99 mTc, and 77Br radioisotopes as 0.72, 1.18, 0.39, 0.57 and 0.46, respectively and the number of DSB for 125I as 0.73.
Using the MOCA7b code, Charlton and Humm calculated the number of DSBs of the DNA molecule caused by the Auger electrons of two different spectra of the 125I radioisotope as 0.90 and 0.65 per decay, respectively. They also concluded that at least 100 eV energy was needed to cause a significant damage to the DNA molecule.
Friedland et al., and Friedland et al., using PARTRAC code showed by increasing the distance between two SSBs for being scored as a DSB from 2 to 10 bp enhanced the DSB yield almost by a factor of 3. Furthermore, DSB yield increases with increasing LET. For heavier ions (B, C, N, O, Ne, S), the DSB yields are lower than for helium ions of the same LET.
It is 17 years after presenting of the atomic model by Pomplun, another volumetric model of the DNA molecule was used by Bernal and Liendo. The same model was also used by Raisali et al., and Semsarha et al.
Bernal and Liendo evaluated the ability of PENELOPE code in nano-dosimetry calculation for a number of radioisotopes using this model. The calculated SSB and DSB values were 20%–76% and 50%–60% lower than those reported in other studies. They attributed this significant decline to the inability of PENELOPE code in tracing low-energy electrons and they proposed that the energy range of PENELOPE code must be expanded up to 10 eV energy in order to calculate DNA damage caused by the electrons.
Raisali et al. used this volumetric model and used the Geant4 code to calculate the Auger electrons-induced SSBs and DSBs after exposure to 123I and 125I radioisotopes.
Considering the foregoing, the Auger electrons are capable of causing damage to the cells and DNA molecules. Therefore, a comprehensive dosimetry assessment must be carried out on the Auger electrons.
Using Geant4-DNA, calculation of SSBs and DSBs caused by low energy electrons were performed by Raisali et al., (Auger electrons of 123I and 125I) and Semsarha et al., (Monoenergetic electrons 1-20 keV). In the present study, dosimetry calculations were performed to evaluate the number of SSBs and DSBs occurring by radioisotopes commonly used in nuclear medicine including 123I, 125I, 99 mTc, 67Ga, 201Tl and 111In, as well as two therapeutic radioisotopes of 131I and 211At on a nanometer scale using Geant4-DNA simulation tool. Despite the lack of information, for some of the radioisotopes the results were compared with the results of other codes as well as the experimental measurements.
The selected radioisotopes have been widely used in the diagnosis and treatment of diseases. 201Tl is used for assessing heart function such as assessing cardiac systolic function and predicting prognosis in patients with cardiovascular disease. 123I is the most suitable isotope of iodine for the diagnostic study of thyroid diseases. Recently it was used to identify myocardial sympathetic denervation patterns in patients with Parkinson's disease. 99mTc is used for imaging and functional studies of brain, myocardium, thyroid, lungs, liver, gallbladder, kidneys, skeleton, blood, and tumors. Approximately 85% of diagnostic imaging procedures in nuclear medicine use this isotope as radioactive tracer. 111In can be used to bind antibodies, peptides, other targeted molecules or cells for imaging and treating cancers. 67Ga is used to image inflammation, chronic infections and tumors. Recently, it was used for detecting infected lower limb prostheses. 125I has uses in biological assays, nuclear medicine imaging and in radiation therapy as brachytherapy to treat a number of conditions, including prostate cancer, uveal melanomas, and pediatric skull base tumors. 131I can be used in medical therapies as a treatment tool. It is most commonly used in the treatment of hyperthyroidism due to Graves disease or a nodule in the thyroid gland. 211At has the most prospective as an alpha emitter for targeted radiotherapy such as treatment of compartmental tumors.
The present study also used two new volumetric model, which are similar to the volumetric model presented by Raisali et al., and Semsarha et al. Two different geometric shapes were considered for the sugar-phosphate groups in the DNA molecule in these two models. The credibility of these simple volumetric models has been confirmed by simulation results, experimental results, and the results of the atomic model proposed by Pomplun. Therefore, these models can be used to reduce the computational time without compromising the accuracy of calculations compared to atomic models, which have more details. Other innovations in this study are the calculation of SSBs and DSBs due to widely-used Auger electron-emitting diagnostic radioisotopes as well as therapeutic radioisotopes emitting alpha and beta particles on a nano-scale measurement using Geant4-DNA simulation in the DNA molecule and to perform a comparison between the effects of diagnostic and therapeutic radioisotopes.
| Materials and Methods|| |
In the present study, two geometric models of the DNA molecule were simulated to calculate the mean number of SSBs and DSBs. These two models are similar to the model used by Bernal and Liendo, Raisali et al., and Semsarha et al., [Figure 1], with the difference that each phosphate-sugar group is in the spherical shape (Model No. 1), and parallelepiped shape (Model No. 2). The two models are indicated in [Figure 2] and [Figure 3]. The geometric model of the DNA molecule considered by Raisali et al., consists of 41 bp (Base pair) (82 nucleotides) with 123I atom being located in 21st bp. This position is the location of the IdUrd.
|Figure 1: The volumetric model of the DNA molecule used by Bernal and Liendo, Raisali et al., and Semsarha et al., (10 base pair of 41 base pair)|
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|Figure 2: Volumetric model of the DNA molecule (spherical sugar- phosphate groups) (Model No. 1)|
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|Figure 3: Volumetric model of the DNA molecule (parallelepiped sugar-phosphate group) (Model No. 2)|
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As shown in [Figure 1], each sugar-phosphate group is simulated in volumes of height of 0.33 nm with an internal radius of 0.5 nm and an external radius of 1.185 nm. Each nucleotide contains a sugar-phosphate group and its related base, in such way that the volume of each sugar-phosphate group is 0.24 nm3. This volume is the total volume of atoms of one sugar-phosphate group. All volumes are filled with water. Considering that the spiral of the DNA molecule spins 360° after 10 nucleotide pairs, an angle of 36° is intended to simulate the rotational angle for each nucleotide.
In this study, the new models were used considering the fact that the shape of phosphate-sugar groups can vary in different DNA molecules. Cylinders, spheres, and parallelepipeds was simulated with the same volume of 0.24 nm3 used by Bernal and Liendo and Raisali et al., (spherical radius: 0.385 nm and parallelepiped sides of: 0.5, 0.5, 0.96 nm and cylinder radius of 0.5 and height of 6.5 nm). The cylinder dimensions, which play the role of the axis of DNA molecule, are exactly the same dimensions used in previous studies Bernal and Liendo and Raisali et al. All volumes are placed inside an air-filled cube as world, with 10-μm sides.
A 10-micron side cube is large enough for the establishment of full scattering conditions, because the range of the low energy electrons is very low in water.
Both DNA molecule models consist of 41 bp (82 nucleotides). Each nucleotide contains a related sugar-phosphate group. The two models are shown in [Figure 2] and [Figure 3]. In the present study, two different models of DNA molecules were simulated to investigate the effect of the geometric shape of phosphate-sugar groups on DNA molecular breaks. All volumes were defined to be water-based. To define the physics of the problem, the physical model of G4EmDNAPhysics was used. Particle emissions were assumed to be isotropic.
[Table 1] shows the average energy of emitted electrons in keV and their yield per decay for the nuclear medicine radionuclides used in simulation. Data of [Table 1] are taken from the AAPM Nuclear Medicine Task Group Report. Furthermore, [Table 2] shows the average energy of emitted electrons and Alpha particles in keV and their yield per decay for the therapeutic radionuclides of 131I (beta + Auger + CK emitter) and 211At (alpha + Auger + CK emitter),,
|Table 1: The average energy of emitted electrons in keV and their yield per decay for the diagnostic radionuclides used in the Monte Carlo-based simulation|
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|Table 2: The average energy of emitted electrons and alpha particles in keV and their yield per decay for the therapeutic radionuclides of 131I (beta + Auger + CK emitter) and 211At (alpha + Auger + CK emitter),,|
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Considering the low energy and short range of the Auger electrons, the radioisotope intracellular position is very important in calculating their dose rate. In practice, radioisotope atoms are placed in 21st bp position at a distance of 0.57 nm from the central axis. The distance between the 125I atom in 125IdUrd and the central axis of DNA was estimated as 0.57 nm by Chen et al.,. Raisali et al., also considered this position for radioisotopes and in order to make a comparison, the same position was selected in the present study too.
Calculations of DNA chain breaks
Due to the interactions of ionizing rays-DNA molecule, various DNA molecule damages occur. In order to determine the number of breaks, the present study investigated the energy absorption in sugar-phosphate volume. Thus, if energy absorbed in the sugar-phosphate group of the DNA molecule is more than the threshold energy value, a break will occur in the DNA molecule chain. This break is called SSB. If the distance between the two SSBs located on the two chains running opposite to each other, is less than the threshold value, then it is considered as a DSB. In this study, the threshold values for SSB and DSB due to direct and indirect effects of DNA molecules was selected similar to the threshold values used by Raisali et al., and Bernal and Liendo and Pomplun. The threshold energy value for SSB due to direct effects was 10.79 eV (This is the first energy ionization of the water molecule defined in the Geant4 Interaction Library, i.e., the threshold energy for detaching the electron from the 1b1 layer) and the threshold energy value for SSB due to indirect effects was 17eV. On average, this energy value is required to produce a radical pair in water. In addition, the threshold distance between two SSBs for being scored as a DSB was considered to be 10 bp.
Given that the simulated DNA molecule has 41 bp and the Auger electrons range is short, the very small threshold distance (<10 bp), results in a significant reduction in the number of DSBs, and a very large threshold (>10 bp) does not have a significant effect on the number of DSBs.
On how to calculate SSBs or DSBs, according to [Table 1] and [Table 2], separately, SSB or DSB for each process, per decay were calculated, then multiplied by the respective yield and eventually the sum of SSBs or DSBs of all the processes were calculated. This is the final reported value of SSB and DSB for each radioisotope.
| Results and Discussion|| |
Calculation of single-strand breaks and double-strand breaks
The results of calculating SSB and DSB caused by the direct and indirect effects for the six commonly used diagnostic radioisotopes of 99 mTc, 67Ga, 201Tl, 111In, 123I, and 125I, as well as the two therapeutic radioisotopes of 131I (beta + Auger + CK emitter) and 211At (alpha + Auger + CK emitter) are presented in [Table 3] and [Table 4], respectively. These results are calculated from average of 10,000 decays and reported for one decay. The selection of 10,000 decays per simulation was sufficient to ensure that the relative standard deviation of the results is <1%.
|Table 3: Average number of single-strand breaks and double-strand breaks per decay caused by the direct effects of the auger electrons, beta and alpha particles for six diagnostic radioisotopes and the two therapeutic radioisotopes for two different geometric models of sugar-phosphate groups|
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|Table 4: Average number of single-strand breaks and double-strand breaks per decay caused by the indirect effects of the Auger electrons, beta and alpha particles for six diagnostic radioisotopes and the two therapeutic radioisotopes for two different geometric models of sugar-phosphate groups|
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The mean difference between SSBs and DSBs of Model No. 1 (spherical) and Model No. 2 (parallelepiped) for the eight radioisotopes is equal to 16.87% and 25.75%, respectively. This difference is due to the effect of different geometric shapes used in both models. Considering the calculated SSBs and DSBs [Table 3], the following inequalities can be written for the above radioisotopes:
SSB values 201Tl >125I >211At >123I >111In >99 mTc >67Ga >131I
DSB values 125I >201Tl >123I >211At >111In >99 mTc >67Ga >131I
SSB values 201Tl >125I >123I >211At >111In >67Ga >99 mTc >131I
DSB values 125I >201Tl >123I >211At >67Ga >99mTc >111In >131I
Considering the above inequalities, 201Tl and 125I have had the greatest rate of SSBs and DSBs per decay in both simulation models. Also, 99 mTc and 67Ga, among the diagnostic radioisotopes had the least effect. According to the emission spectrum of the studied radioisotopes [Table 1], 201Tl and 125I had the highest decay yields (38 and 21.5 electron per decay), respectively. As shown in [Table 2], 131I had the lowest decay yields (1.59 electron per decay). It should be noted that in addition to decay yields, the amount of particles energy also influences the breaks rate. For example, 211At has low decay yields (6.21 electron per decay) but the proper energy of its electrons and alpha particles, caused a moderate impact on the breaks rate.
The mean energy of particles emitted from 125I is less than that of 201Tl, which results in the absorption of 125I electrons at shorter distances than those emitted from 201Tl in the sugar-phosphate group of the DNA molecule, which in turn leads to higher DSBs for 125I in comparison with 201Tl.
The 131I therapeutic radioisotope almost did not have any effect on the SSBs and DSBs in both models, and the 211At therapeutic alpha-emitting radioisotope had a moderate effect in this regard. This reveals the significance of dosimetry calculation and the study of the effects of the Auger electron-emitting diagnostic radioisotopes. [Table 5] shows the results of studies previously carried out by Raisali et al., Pomplun and Ftacnikova and Bohm who calculated the SSBs and DSBs caused by the Auger electrons for 123I and 125I radioisotopes.
|Table 5: The mean number of single-strand breaks and double-strand breaks caused by the direct effects of the Auger electrons per decay of the two radioisotopes of I and I, calculated by Raisali et al.,, Pomplun and Ftacnikova and Bohm|
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The SSBs and DSBs calculated by Raisali et al., Pomplun and Ftacnikova and Bohm are lower than those obtained in the present study [Table 3], which may be attributed to differences in calculation methods:
i. The volume of the sugar-phosphate groups proposed in the present study was equal to the same volume considered by Raisali et al.; so, different geometric shapes of sugar-phosphate group can be considered as a factor leading to the difference in results. The effect of the sugar-phosphate group geometric shape varies in different energies, and this comparison demonstrates the importance of the effect of the geometric shape on the breaks of DNA molecules
ii. Differences in the radioisotope emission spectrum. For example, in the spectrum used for 123I and 125I radioisotopes Raisali et al., the yield per decay is considered to be 2.26 and 8.12, respectively, for Auger NXY electrons; however, the same values were reported to be 6.54 and 10.9 in the case of the spectrum used in the present study
iii. Differences in the simulation code (MOCA8b and ETRACK), the difference in the threshold energy value of SSBs (17.6 eV), and the different position of radioisotopes are considered as the main reasons for the difference between the results.
Comparison of 123I and 125I radioisotopes showed that the mean SSB and DSB values of the 125I in the spherical model were respectively 1.65 and 1.66 times more effective than 123I, and in cubic model were 1.68 and 1.66 times more effective than the 123I radioisotope. Raisali et al., (cylindrical model) reported that the mean of SSB and DSB values of 125I radioisotope are 1.5 times more effective than 123I. Also, the β-particles of 131I, due to their energy and high range, had not any effect on the SSB and DSB of the DNA molecule.
The mean difference between SSBs and DSBs of Model No. 1 (spherical) and Model No. 2 (parallelepiped) for 8 radioisotopes is equal to 15.07% and 22.63%, respectively.
For all the radioisotopes, for both geometric models, the number of breaks caused by indirect effects is less than direct effects, and even the number of DSBs caused by indirect effects for most of the radioisotopes is zero. The lower the number of breaks due to indirect effects than direct effects is naturally due to the higher threshold that is considered for breaks by indirect effects. Considering the calculated SSBs and DSBs [Table 4], the following inequalities can be written for the above radioisotopes:
SSB values 125I >201Tl >211At >123I >111In >67Ga >99 mTc >131I
DSB values 125I >201Tl >211At >123I =111In =67Ga =99 mTc =131I
SSB values 125I >201Tl >123I >211At >111In >99 mTc >67Ga >131I
DSB values 211At >125I >201Tl >123I =111In = 67Ga =99 mTc =131I
Considering the above inequalities, 125I almost in both simulation models has the greatest impact on the number of SSBs and DSBs due to indirect effects. Considering the total effects of direct and indirect [Table 3] and [Table 4], 201Tl and 125I, had the greatest impact on the number of SSBs and DSBs, respectively.
Considering the threshold of DNA molecule chain breaks to induce cell death, we can evaluate the effects of cellular death induced by the Auger electrons of the widely used diagnostic radioisotopes. For example, 125I, as one of the most effective radioisotopes on the DNA molecule chain breaks, leads to an average SSB and DSB number of 3.45 and 1.11 per decay [Table 6], respectively (in the spherical model). Therefore, a total of more than 43,000 decay of this radioisotope should occur to reach the threshold value for the induction of cell death as apoptosis. This conclusion has been made using a few approximations, and considering the repair potential in different cells, this conclusion needs to be corrected. To achieve definitive results, further calculations and laboratory researches need to be carried out on the rate of SSBs and DSBs of the DNA molecule caused by the Auger electron of the diagnostic radioisotopes.
|Table 6: Number of mean single-strand breaks and double-strand breaks caused by the direct and indirect effects of 125I auger electrons calculated by Geant4-DNA, and a comparison with Raisali et al., as well as experimental results|
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Validation of simulation results
Among the diagnostic and therapeutic radioisotopes located on the base thymidine position of the 21st bp of DNA molecule, only the SSBs and DSBs of the 125I radioisotope (as 125IdUrd radiopharmaceutical) have been investigated in vitro. IdUrd is a radiopharmaceutical that carries 123I or 125I and is located on the base thymidine position of the DNA molecule. The number of SSBs and DSBs was not measured directly in case of 123I radioisotope (as 123IdUrd radiopharmaceuticals).
Thus, out of the radioisotopes that are simulated in this study, only the simulation results of the 125I, a diagnostic radioisotope, can be compared with experimental results. This position (21st bp of DNA molecule) was selected in the present research so as to compare the results with the results of Raisali et al.'s research, in which the same position was also selected. Another reason for choosing such position for the remaining radioisotopes, regardless of the possibility of labeling the above radioisotopes with the above drug, was simply to select the same position for all radioisotopes in order to compare their effects on the molecular chain breaks. Overall, it can be stated that the simulation results of these diagnostic and therapeutic radioisotopes can provide useful information to achieve a better understanding of their effects on DNA molecule. Also, if these radioisotopes are labeled with the above drug (IdUrd) in the future, the results of this research will be valuable.
LeMotte and Little measured the number of 125I–induced SSBs and DSBs in human diploid fibroblasts using experimental methods.
[Table 6] shows the results of total SSBs and DSBs induced by direct and indirect effects obtained in this study, the results of Raisali et al., together with the experimental results of LeMotte and Little.
The data presented in [Table 6] show that the simulation results of both models No. 1 and 2 are almost in good agreement with the simulation results of Raisali et al., and the experimental results of LeMotte and Little. Roots et al., also suggested that the measured values by LeMotte and Little should be reduced by 30% to correct the number of SSBs measured by this experimental method. Thus, the number of SSBs would be equal to 3.01 accordingly and our results would be in better agreement with the SSBs of the experimental results.
| Conclusion|| |
The aim of the present study was to conduct a comprehensive assessment on the effects of widely-used diagnostic nuclear medicine radioisotopes as well as therapeutic radioisotopes on a nano-scale measurement using Geant4-DNA simulation. The present study investigated the effects of these radioisotopes on SSBs and DSBs in the DNA molecule. The results showed that 201Tl and 125I had the maximum effect on DNA by inducing number of SSBs and DSBs, while the therapeutic radioisotope of 131I (beta + Auger + CK emitter) almost had no effect on the rate of induction of strand breaks and therapeutic radioisotope of 211At (alpha + Auger + CK emitter) had the moderate effect. These results demonstrated the importance of performing nanoscale dosimetry calculation on these diagnostic radioisotopes, and also prove the potential of using these radioisotopes in the radiation therapy. In this study, two models with two different shapes of sugar-phosphate group were simulated to investigate the effect of geometric shape of sugar-phosphate groups on the rate of DNA SSBs and DSBs. The results of the two models were also compared with each other as well as with the results of others. The results revealed that different geometric shapes of sugar-phosphate groups could have a significant effect on the rate of SSBs and DSBs, even if the sugar-phosphate groups were considered to be of the same volume.
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Conflicts of interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]