Journal of Medical Physics
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Year : 1986  |  Volume : 11  |  Issue : 3  |  Page : 352

Photodynamic Effect On He-La Tumour Cell Line With Lasers And Photosensitizes



Correspondence Address:
S Ganesan


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Photodynamic therapy (PDT) of its specificity and its capability of irradiation and regression of tumour even in internal organs. The technique involves laser irradiation of photosensitizor which specifically and preferentially binds on tumour cells. This paper reports the results of one such investigation with He-La tumour cell line as target and lasers as light source and a few dyes as sensitizer. The laser used are ultraviolet Nitrogen laser (100 KW nower, 10 ns pulse duration, I Uz repetition rate) and He-Ne (15Mw). The sensitizers used are HpD, Acridine orange, etc. The cell damage is measured by counting viable cells. The investigation shows that cell damage is decisively higher when laser and sensitizor are used in conjunction then when each is used individually. Results of cell damage for each laser as a function the nature of sensitizer, its concentration, laser energy dosage and incubation time are presented.


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